Novel Nanobiocatalyst Constituted by Lipase from Burkholderia cepacia Immobilized on Graphene Oxide Derived from Grape Seed Biochar

The present research aims to study the process of immobilization lipase from Burkholderia cepacia by physical adsorption on graphene oxide derived (GO) grape seed biochar. Additionally, modified Hummers method was used obtain oxide. Moreover, Fourier transform infrared spectroscopy, Raman spectrum, X-ray diffraction, and point zero charge were for characterization GO. influences pH, temperature, enzyme/support concentration catalytic activity evaluated immobilized biocatalyst. best found (543 ± 5 U/g support) in pH 4.0. Considering biochemical properties, optimal temperature 3.0 50 °C, respectively, Reusability studies exhibited that well kept 60% its original after cycles reuse. Overall, these results showed high potential obtained biochar lipase, especially application nanobiocatalysts an industrial scale.